Grueneisen relaxation photoacoustic microscopy in vivo
#MMPMID27272096
Ma J
; Shi J
; Hai P
; Zhou Y
; Wang LV
J Biomed Opt
2016[Jun]; 21
(6
): 66005
PMID27272096
show ga
Grueneisen relaxation photoacoustic microscopy (GR-PAM) can achieve optically
defined axial resolution, but it has been limited to ex vivo demonstrations so
far. Here, we present the first in vivo image of a mouse brain acquired with
GR-PAM. To induce the GR effect, an intensity-modulated continuous-wave laser was
employed to heat absorbing objects. In phantom experiments, an axial resolution
of 12.5???m was achieved, which is sixfold better than the value achieved by
conventional optical-resolution PAM. This axial-resolution improvement was
further demonstrated by imaging a mouse brain in vivo, where significantly
narrower axial profiles of blood vessels were observed. The in vivo demonstration
of GR-PAM shows the potential of this modality for label-free and high-resolution
anatomical and functional imaging of biological tissues.
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