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2017 ; 13
(ä): 1145-1167
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Glycoscience@Synchrotron: Synchrotron radiation applied to structural
glycoscience
#MMPMID28684994
Pérez S
; de Sanctis D
Beilstein J Org Chem
2017[]; 13
(ä): 1145-1167
PMID28684994
show ga
Synchrotron radiation is the most versatile way to explore biological materials
in different states: monocrystalline, polycrystalline, solution, colloids and
multiscale architectures. Steady improvements in instrumentation have made
synchrotrons the most flexible intense X-ray source. The wide range of
applications of synchrotron radiation is commensurate with the structural
diversity and complexity of the molecules and macromolecules that form the
collection of substrates investigated by glycoscience. The present review
illustrates how synchrotron-based experiments have contributed to our
understanding in the field of structural glycobiology. Structural
characterization of protein-carbohydrate interactions of the families of most
glycan-interacting proteins (including glycosyl transferases and hydrolases,
lectins, antibodies and GAG-binding proteins) are presented. Examples concerned
with glycolipids and colloids are also covered as well as some dealing with the
structures and multiscale architectures of polysaccharides. Insights into the
kinetics of catalytic events observed in the crystalline state are also presented
as well as some aspects of structure determination of protein in solution.