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10.1007/978-1-4939-4035-6_9 http://scihub22266oqcxt.onion/10.1007/978-1-4939-4035-6_9 C5880289!5880289 !27662873     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27662873 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Methods+Mol+Biol 2017 ; 1468 (ä): 111-20 Nephropedia Template TP {{tp|p=27662873 |t=2017. Global Run-On Sequencing (GRO-Seq) |pdf=|usr=}}{{27662873 }} gab.com Text Global Run-On Sequencing (GRO-Seq) JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=27662873 #FOAvip Transcription occurring at gene loci results in accumulation of mature RNA molecules (i.e., mRNAs) that can be easily assayed by RT-PCR or RNA sequencing. However, the steady-state level of RNA does not accurately mirror transcriptional activity per se. In fact, RNA stability plays a major role in determining the relative abundance of any given RNA molecule. Here, I describe a protocol of Nuclear Run-On assay coupled to deep sequencing to assess real-time transcription from engaged RNA polymerase. Mapping nascent transcripts at the genome-wide scale provides a reliable measure of transcriptional activity in mammalian cells and delivers a high-resolution map of coding and noncoding transcripts that is especially useful for annotation and quantification of short-lived RNA molecules. Twit Text FOAVip Global Run-On Sequencing (GRO-Seq) ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=27662873 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27662873 #FOAvip English Wikipedia <ref>{{Cite pmid|27662873 }}</ref> Global Run-On Sequencing (GRO-Seq) #MMPMID27662873 Gardini A Methods Mol Biol 2017[]; 1468 (ä): 111-20 PMID27662873 show ga Transcription occurring at gene loci results in accumulation of mature RNA molecules (i.e., mRNAs) that can be easily assayed by RT-PCR or RNA sequencing. However, the steady-state level of RNA does not accurately mirror transcriptional activity per se. In fact, RNA stability plays a major role in determining the relative abundance of any given RNA molecule. Here, I describe a protocol of Nuclear Run-On assay coupled to deep sequencing to assess real-time transcription from engaged RNA polymerase. Mapping nascent transcripts at the genome-wide scale provides a reliable measure of transcriptional activity in mammalian cells and delivers a high-resolution map of coding and noncoding transcripts that is especially useful for annotation and quantification of short-lived RNA molecules. |Animals [MESH] |Chromosome Mapping [MESH] |DNA-Directed RNA Polymerases/metabolism [MESH] |High-Throughput Nucleotide Sequencing/*methods [MESH] |Humans [MESH] |RNA Stability [MESH] |RNA, Long Noncoding/chemistry/genetics [MESH] |RNA/chemistry/*genetics [MESH] |Sequence Analysis, RNA/*methods [MESH] |Transcription Initiation Site [MESH]Global Run-On Sequencing (GRO-Seq) (epmc).pdf DeepDyve Pubget Overpricing