Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24136966
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Science
2013 ; 342
(6156
): 357-60
Nephropedia Template TP
Lajoie MJ
; Rovner AJ
; Goodman DB
; Aerni HR
; Haimovich AD
; Kuznetsov G
; Mercer JA
; Wang HH
; Carr PA
; Mosberg JA
; Rohland N
; Schultz PG
; Jacobson JM
; Rinehart J
; Church GM
; Isaacs FJ
Science
2013[Oct]; 342
(6156
): 357-60
PMID24136966
show ga
We describe the construction and characterization of a genomically recoded
organism (GRO). We replaced all known UAG stop codons in Escherichia coli MG1655
with synonymous UAA codons, which permitted the deletion of release factor 1 and
reassignment of UAG translation function. This GRO exhibited improved properties
for incorporation of nonstandard amino acids that expand the chemical diversity
of proteins in vivo. The GRO also exhibited increased resistance to T7
bacteriophage, demonstrating that new genetic codes could enable increased viral
resistance.