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10.1126/science.1241459

http://scihub22266oqcxt.onion/10.1126/science.1241459
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C4924538!4924538!24136966
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suck abstract from ncbi


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pmid24136966      Science 2013 ; 342 (6156): 357-60
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  • Genomically Recoded Organisms Expand Biological Functions #MMPMID24136966
  • Lajoie MJ; Rovner AJ; Goodman DB; Aerni HR; Haimovich AD; Kuznetsov G; Mercer JA; Wang HH; Carr PA; Mosberg JA; Rohland N; Schultz PG; Jacobson JM; Rinehart J; Church GM; Isaacs FJ
  • Science 2013[Oct]; 342 (6156): 357-60 PMID24136966show ga
  • We describe the construction and characterization of a genomically recoded organism (GRO). We replaced all known UAG stop codons in Escherichia coli MG1655 with synonymous UAA codons, which permitted the deletion of release factor 1 and reassignment of UAG translation function. This GRO exhibited improved properties for incorporation of nonstandard amino acids that expand the chemical diversity of proteins in vivo. The GRO also exhibited increased resistance to T7 bacteriophage, demonstrating that new genetic codes could enable increased viral resistance.
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