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2015 ; 10
(2
): 711-716
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Genomic and proteomic investigation of preeclampsia
#MMPMID26622380
Sahin H
; Gunel T
; Benian A
; Onay Ucar E
; Guralp O
; Kilic A
Exp Ther Med
2015[Aug]; 10
(2
): 711-716
PMID26622380
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The aim of this study was to use proteomic and transcriptomic approaches to
examine differences in protein and gene expression in maternal plasma between
normal and preeclamptic pregnancies. Preeclampsia and control groups were
compared with respect to the expression of CD34 and CD133 genes by quantitative
polymerase chain reaction (qPCR) and heat shock protein (Hsp)27 and 70 by western
blotting in blood samples from the pregnant women. Blood samples were obtained at
gestational week 12-14 from 65 healthy pregnant women. Fetal DNA was isolated
from the maternal blood and CD34 and CD133 were amplified by qPCR. Western blot
analysis was used to examine the expression levels of Hsp27 and Hsp70 proteins.
The analysis of CD133 by qPCR was unsuccessful in 7 women as the levels of fetal
DNA were in the collected maternal blood samples were insufficient. Measurements
of CD34 and CD133 were performed in 57 and 50 women, respectively. Preeclampsia
developed in 6 (10.5%) of 57 women. qPCR results of 8 healthy pregnant women were
used for the calibration of CD34 and CD133 levels, and the results for the
remaining women were compared with the calibration values. CD34 expression was
decreased in 30 (52.6%) and increased in 27 (47.4%) of 57 women. CD133 expression
was decreased in 14 (28%) and increased in 36 (72%) of 50 women. CD34 expression
was increased in 2 (33%) and 25 (49%) and decreased in 4 (66%) and 25 (51%) women
with and without preeclampsia, respectively (P=0.467). CD133 expression was
increased in 4 (66%) and 32 (72%); and decreased in 2 (33%) and 12 (28%) women
with and without preeclampsia, respectively (P=0.756). Western blotting showed
that the expression of Hsp27 and Hsp70 in the maternal serum of the preeclampsia
group was significantly higher than that in the normal pregnancy group. CD34 and
CD133 were found to be inadequate for use in the prediction of preeclampsia.
However, it is noteworthy that CD133 levels were increased in 66 and 72% of women
with and without preeclampsia, respectively. Hsps are expressed under various
pathological conditions. These results suggest that conditions of oxidative
stress increased the Hsp27 and Hsp70 protein levels.
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