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10.1128/MCB.00477-15 http://scihub22266oqcxt.onion/10.1128/MCB.00477-15 C4648815!4648815 !26416877     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26416877 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Cell+Biol 2015 ; 35 (24 ): 4135-46 Nephropedia Template TP {{tp|p=26416877 |t=2015. Genomic Determinants of THAP11/ZNF143/HCFC1 Complex Recruitment to Chromatin |pdf=|usr=}}{{26416877 }} gab.com Text Genomic Determinants of THAP11/ZNF143/HCFC1 Complex Recruitment to Chromatin JO: Mol Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=26416877 #FOAvip The THAP11 and ZNF143 transcription factors recognize overlapping DNA sequences and are reported to exhibit signs of both competitive and cooperative binding. HCFC1 serves as a scaffold protein, bridging interactions between transcription factors, including THAP11 and ZNF143, and transcriptional coregulators. The exact mechanism of how DNA sequences guide the recruitment of the THAP11/ZNF143/HCFC1 complex to chromatin is still controversial. In this study, we use chromosomally integrated synthetic constructs and clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9-mediated approaches in intact cells to elucidate the role of the DNA sequence in the recruitment of this complex and to establish its biological relevance. We show that the ACTACA submotif, shared by both THAP11 and ZNF143, directs the recruitment of THAP11 and HCFC1 to ZNF143-occupied loci. Importantly, its position, spacing, and orientation relative to the ZNF143 core motif are critical for this action. CRISPR-Cas9-mediated alterations of the ACTACA submotif at endogenous promoters recapitulated results obtained with synthetic constructs and resulted in altered gene transcription and histone modifications at targeted promoters. Our in vivo approaches provide strong evidence for the molecular role of the ACTACA submotif in THAP11, ZNF143, and HCFC1 cooperative recruitment to chromatin and its biological role in target gene expression. Twit Text FOAVip Genomic Determinants of THAP11/ZNF143/HCFC1 Complex Recruitment to Chromatin ????Mol Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=26416877 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26416877 #FOAvip English Wikipedia <ref>{{Cite pmid|26416877 }}</ref> Genomic Determinants of THAP11/ZNF143/HCFC1 Complex Recruitment to Chromatin #MMPMID26416877 Vinckevicius A ; Parker JB ; Chakravarti D Mol Cell Biol 2015[Dec]; 35 (24 ): 4135-46 PMID26416877 show ga The THAP11 and ZNF143 transcription factors recognize overlapping DNA sequences and are reported to exhibit signs of both competitive and cooperative binding. HCFC1 serves as a scaffold protein, bridging interactions between transcription factors, including THAP11 and ZNF143, and transcriptional coregulators. The exact mechanism of how DNA sequences guide the recruitment of the THAP11/ZNF143/HCFC1 complex to chromatin is still controversial. In this study, we use chromosomally integrated synthetic constructs and clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9-mediated approaches in intact cells to elucidate the role of the DNA sequence in the recruitment of this complex and to establish its biological relevance. We show that the ACTACA submotif, shared by both THAP11 and ZNF143, directs the recruitment of THAP11 and HCFC1 to ZNF143-occupied loci. Importantly, its position, spacing, and orientation relative to the ZNF143 core motif are critical for this action. CRISPR-Cas9-mediated alterations of the ACTACA submotif at endogenous promoters recapitulated results obtained with synthetic constructs and resulted in altered gene transcription and histone modifications at targeted promoters. Our in vivo approaches provide strong evidence for the molecular role of the ACTACA submotif in THAP11, ZNF143, and HCFC1 cooperative recruitment to chromatin and its biological role in target gene expression. |Base Sequence [MESH] |Binding Sites/genetics [MESH] |Cell Line [MESH] |Chromatin/genetics [MESH] |Clustered Regularly Interspaced Short Palindromic Repeats [MESH] |DNA-Binding Proteins/genetics [MESH] |Gene Expression [MESH] |HEK293 Cells [MESH] |HeLa Cells [MESH] |Host Cell Factor C1/*genetics [MESH] |Humans [MESH] |Multiprotein Complexes/*genetics [MESH] |Nucleotide Motifs/*genetics [MESH] |Promoter Regions, Genetic/genetics [MESH] |RNA Editing/genetics [MESH] |Repressor Proteins/*genetics [MESH] |Trans-Activators/*genetics [MESH]Genomic Determinants of THAP11/ZNF143/HCFC1 Complex Recruitment to Chr(epmc).pdf DeepDyve Pubget Overpricing