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2015 ; 6
(7
): e1831
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Genome-editing tools for stem cell biology
#MMPMID26203860
Vasileva EA
; Shuvalov OU
; Garabadgiu AV
; Melino G
; Barlev NA
Cell Death Dis
2015[Jul]; 6
(7
): e1831
PMID26203860
show ga
Human pluripotent stem cells provide a versatile platform for regenerative
studies, drug testing and disease modeling. That the expression of only four
transcription factors, Oct4, Klf4, Sox2 and c-Myc (OKSM), is sufficient for
generation of induced pluripotent stem cells (iPSCs) from differentiated somatic
cells has revolutionized the field and also highlighted the importance of OKSM as
targets for genome editing. A number of novel genome-editing systems have been
developed recently. In this review, we focus on successful applications of
several such systems for generation of iPSCs. In particular, we discuss
genome-editing systems based on zinc-finger fusion proteins (ZFs), transcription
activator-like effectors (TALEs) and an RNA-guided DNA-specific nuclease, Cas9,
derived from the bacterial defense system against viruses that utilizes clustered
regularly interspaced short palindromic repeats (CRISPR).
|*Clustered Regularly Interspaced Short Palindromic Repeats
[MESH]
free
free
free
