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10.1038/ncomms15114 http://scihub22266oqcxt.onion/10.1038/ncomms15114 C5418625!5418625!28466845     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28466845.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Commun 2017 ; 8 (ä): ä Nephropedia Template TP {{tp|p=28466845|t=2017. Genetic variation and RNA structure regulate microRNA biogenesis |pdf=|usr=}}{{28466845}} gab.com Text Genetic variation and RNA structure regulate microRNA biogenesis JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28466845 #FOAvip MiRNA biogenesis is highly regulated at the post-transcriptional level; however, the role of sequence and secondary RNA structure in this process has not been extensively studied. A single G to A substitution present in the terminal loop of pri-mir-30c-1 in breast and gastric cancer patients had been previously described to result in increased levels of mature miRNA. Here, we report that this genetic variant directly affects Drosha-mediated processing of pri-mir-30c-1 in vitro and in cultured cells. Structural analysis of this variant revealed an altered RNA structure that facilitates the interaction with SRSF3, an SR protein family member that promotes pri-miRNA processing. Our results are compatible with a model whereby a genetic variant in pri-mir-30c-1 leads to a secondary RNA structure rearrangement that facilitates binding of SRSF3 resulting in increased levels of miR-30c. These data highlight that primary sequence determinants and RNA structure are key regulators of miRNA biogenesis. Twit Text FOAVip Genetic variation and RNA structure regulate microRNA biogenesis ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28466845 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28466845 #FOAvip English Wikipedia <ref>{{Cite pmid|28466845}}</ref> Genetic variation and RNA structure regulate microRNA biogenesis #MMPMID28466845 Fernandez N; Cordiner RA; Young RS; Hug N; Macias S; Cáceres JF Nat Commun 2017[]; 8 (ä): ä PMID28466845show ga MiRNA biogenesis is highly regulated at the post-transcriptional level; however, the role of sequence and secondary RNA structure in this process has not been extensively studied. A single G to A substitution present in the terminal loop of pri-mir-30c-1 in breast and gastric cancer patients had been previously described to result in increased levels of mature miRNA. Here, we report that this genetic variant directly affects Drosha-mediated processing of pri-mir-30c-1 in vitro and in cultured cells. Structural analysis of this variant revealed an altered RNA structure that facilitates the interaction with SRSF3, an SR protein family member that promotes pri-miRNA processing. Our results are compatible with a model whereby a genetic variant in pri-mir-30c-1 leads to a secondary RNA structure rearrangement that facilitates binding of SRSF3 resulting in increased levels of miR-30c. These data highlight that primary sequence determinants and RNA structure are key regulators of miRNA biogenesis. äGenetic variation and RNA structure regulate microRNA biogenesis (epmc).pdf DeepDyve Pubget Overpricing