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10.1038/nchembio.2160 http://scihub22266oqcxt.onion/10.1038/nchembio.2160 C5215917!5215917 !27571478     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27571478 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Chem+Biol 2016 ; 12 (10 ): 776-778 Nephropedia Template TP {{tp|p=27571478 |t=2016. Genetic code expansion in the mouse brain |pdf=|usr=}}{{27571478 }} gab.com Text Genetic code expansion in the mouse brain JO: Nat Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=27571478 #FOAvip Site-specific incorporation of non-natural amino acids into proteins, via genetic code expansion with pyrrolysyl tRNA synthetase (PylRS) and tRNA(Pyl)CUA pairs (and their evolved derivatives) from Methanosarcina sp., forms the basis of powerful approaches to probe and control protein function in cells and invertebrate organisms. Here we demonstrate that adeno-associated viral delivery of these pairs enables efficient genetic code expansion in primary neuronal culture, organotypic brain slices and the brains of live mice. Twit Text FOAVip Genetic code expansion in the mouse brain ????Nat Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=27571478 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27571478 #FOAvip English Wikipedia <ref>{{Cite pmid|27571478 }}</ref> Genetic code expansion in the mouse brain #MMPMID27571478 Ernst RJ ; Krogager TP ; Maywood ES ; Zanchi R ; Beránek V ; Elliott TS ; Barry NP ; Hastings MH ; Chin JW Nat Chem Biol 2016[Oct]; 12 (10 ): 776-778 PMID27571478 show ga Site-specific incorporation of non-natural amino acids into proteins, via genetic code expansion with pyrrolysyl tRNA synthetase (PylRS) and tRNA(Pyl)CUA pairs (and their evolved derivatives) from Methanosarcina sp., forms the basis of powerful approaches to probe and control protein function in cells and invertebrate organisms. Here we demonstrate that adeno-associated viral delivery of these pairs enables efficient genetic code expansion in primary neuronal culture, organotypic brain slices and the brains of live mice. |Amino Acids/*chemistry/*genetics/metabolism [MESH] |Amino Acyl-tRNA Synthetases/*metabolism [MESH] |Animals [MESH] |Brain/*cytology/*metabolism [MESH] |Dependovirus/genetics [MESH] |Genetic Code/*genetics [MESH] |Methanosarcina/genetics [MESH] |Mice [MESH] |Molecular Structure [MESH]Genetic code expansion in the mouse brain (epmc).pdf DeepDyve Pubget Overpricing