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10.1038/bonekey.2014.65 http://scihub22266oqcxt.onion/10.1038/bonekey.2014.65 C4162467!4162467 !25228983     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25228983 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Bonekey+Rep 2014 ; 3 (ä): 570 Nephropedia Template TP {{tp|p=25228983 |t=2014. Generation and culture of osteoclasts |pdf=|usr=}}{{25228983 }} gab.com Text Generation and culture of osteoclasts JO: Bonekey Rep http://www.kidney.de/pget.php?&tw=1&pmid=25228983 #FOAvip Osteoclasts are highly specialized cells of haematopoietic lineage that are uniquely responsible for bone resorption. In the past, osteoclasts were isolated as mature cells from chicken long bones, or were generated using osteoblasts or stromal cells to induce osteoclast formation in total bone marrow from mice or rabbits. The Copernican revolution in osteoclast biology began with the identification of macrophage-colony stimulating factor (M-CSF) and receptor activator NF?B-ligand (RANKL ) as the key regulators of osteoclast formation, fusion and function. The availability of recombinant human and mouse M-CSF and RANKL has enabled researchers to reliably generate osteoclasts from primary monocyte/macrophage cells as well as from cell lines such as RAW 264.7. This article summarizes the most commonly used procedures for the isolation, generation and characterization of human, rodent and chicken osteoclasts in vitro. Lists of further reading and recommendations are included to facilitate a successful application by the reader. Twit Text FOAVip Generation and culture of osteoclasts ????Bonekey Rep http://www.kidney.de/pget.php?&tw=1&pmid=25228983 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25228983 #FOAvip English Wikipedia <ref>{{Cite pmid|25228983 }}</ref> Generation and culture of osteoclasts #MMPMID25228983 Marino S ; Logan JG ; Mellis D ; Capulli M Bonekey Rep 2014[]; 3 (ä): 570 PMID25228983 show ga Osteoclasts are highly specialized cells of haematopoietic lineage that are uniquely responsible for bone resorption. In the past, osteoclasts were isolated as mature cells from chicken long bones, or were generated using osteoblasts or stromal cells to induce osteoclast formation in total bone marrow from mice or rabbits. The Copernican revolution in osteoclast biology began with the identification of macrophage-colony stimulating factor (M-CSF) and receptor activator NF?B-ligand (RANKL ) as the key regulators of osteoclast formation, fusion and function. The availability of recombinant human and mouse M-CSF and RANKL has enabled researchers to reliably generate osteoclasts from primary monocyte/macrophage cells as well as from cell lines such as RAW 264.7. This article summarizes the most commonly used procedures for the isolation, generation and characterization of human, rodent and chicken osteoclasts in vitro. Lists of further reading and recommendations are included to facilitate a successful application by the reader. äGeneration and culture of osteoclasts (epmc).pdf DeepDyve Pubget Overpricing