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Gene Set Analysis: A Step-By-Step Guide #MMPMID26059482
Mooney MA; Wilmot B
Am J Med Genet B Neuropsychiatr Genet 2015[Oct]; 168 (7): 517-27 PMID26059482show ga
To maximize the potential of genome-wide association studies, many researchers are performing secondary analyses to identify sets of genes jointly associated with the trait of interest. Although methods for gene-set analyses (GSA), also called pathway analyses, have been around for more than a decade, the field is still evolving. There are numerous algorithms available for testing the cumulative effect of multiple SNPs, yet no real consensus in the field about the best way to perform a GSA. This paper provides an overview of the factors that can affect the results of a GSA, the lessons learned from past studies, and suggestions for how to make analysis choices that are most appropriate for different types of data.