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2010 ; 33
(2
): 81-94
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Gene expression in oligodendroglial tumors
#MMPMID20966545
Shaw EJ
; Haylock B
; Husband D
; du Plessis D
; Sibson DR
; Warnke PC
; Walker C
Anal Cell Pathol (Amst)
2010[]; 33
(2
): 81-94
PMID20966545
show ga
BACKGROUND: Oligodendroglial tumors with 1p/19q loss are more likely to be
chemosensitive and have longer survival than those with intact 1p/19q, but not
all respond to chemotherapy, warranting investigation of the biological basis of
chemosensitivity. METHODS: Gene expression profiling was performed using
amplified antisense RNA from 28 oligodendroglial tumors treated with chemotherapy
(26 serial stereotactic biopsy, 2 resection). Expression of differentially
expressed genes was validated by real-time PCR. RESULTS: Unsupervised
hierarchical clustering showed clustering of multiple samples from the same case
in 14/17 cases and identified subgroups associated with tumor grade and 1p/19q
status. 176 genes were differentially expressed, 164 being associated with 1p/19q
loss (86% not on 1p or 19q). 94 genes differed between responders and
non-responders to chemotherapy; 12 were not associated with 1p/19q loss.
Significant differential expression was confirmed in 11/13 selected genes. Novel
genes associated with response to therapy included SSBP2, GFRA1, FAP and RASD1.
IQGAP1, INA, TGIF1, NR2F2 and MYCBP were differentially expressed in
oligodendroglial tumors with 1p/19q loss. CONCLUSION: Gene expression profiling
using serial stereotactic biopsies indicated greater homogeneity within tumors
than between tumors. Genes associated with 1p/19q status or response were
identified warranting further elucidation of their role in oligodendroglial
tumors.
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