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10.21873/invivo.11062 http://scihub22266oqcxt.onion/10.21873/invivo.11062 C5461440!5461440 !28438858     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28438858 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28438858 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       In+Vivo 2017 ; 31 (3 ): 321-328 Nephropedia Template TP {{tp|p=28438858 |t=2017. Functional Roles of Netrin-1 in Osteoblast Differentiation |pdf=|usr=}}{{28438858 }} gab.com Text Functional Roles of Netrin-1 in Osteoblast Differentiation JO: In Vivo http://www.kidney.de/pget.php?&tw=1&pmid=28438858 #FOAvip AIM: Recent studies have demonstrated that netrin-1 plays a vital role in bone metabolism. Previous studies have shown that osteoblasts produce netrin-1 which affects osteoclast differentiation. However, the role of netrin-1 in osteoblast differentiation is not well understood. In this study, we explored the roles of netrin-1 in osteoblasts. MATERIALS AND METHODS: Quantitative reverse-transcriptase polymerase chain reaction (qPCR), RNA interference for netrin receptors, the generation of netrin-1 plasmid, transfection of plasmids, and cell proliferation assay were performed. RESULTS: During osteoblast differentiation by ascorbic acid, netrin-1 expression was significantly decreased. Gene expression related with osteoblast differentiation was down-regulated by netrin-1 treatment. We also found that osteoblast differentiation by bone morphogenetic protein-4 (BMP-4) was inhibited in the presence of recombinant netrin-1. Forced expression of both BMP-4 and netrin-1 significantly decreased alkaline phosphatase expression. On the other hand, Unc5b, neogenin, and A2b which belong to netrin receptors were expressed by osteoblasts. Moreover, alkaline phosphatase expression was significantly decreased by knockdown for the combination of two receptors among these receptors. CONCLUSION: Netrin-1 is involved in the regulation of osteoblast differentiation. Twit Text FOAVip Functional Roles of Netrin-1 in Osteoblast Differentiation ????In Vivo http://www.kidney.de/pget.php?&tw=1&pmid=28438858 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28438858 #FOAvip English Wikipedia <ref>{{Cite pmid|28438858 }}</ref> Functional Roles of Netrin-1 in Osteoblast Differentiation #MMPMID28438858 Sato T ; Kokabu S ; Enoki Y ; Hayashi N ; Matsumoto M ; Nakahira M ; Sugasawa M ; Yoda T In Vivo 2017[May]; 31 (3 ): 321-328 PMID28438858 show ga AIM: Recent studies have demonstrated that netrin-1 plays a vital role in bone metabolism. Previous studies have shown that osteoblasts produce netrin-1 which affects osteoclast differentiation. However, the role of netrin-1 in osteoblast differentiation is not well understood. In this study, we explored the roles of netrin-1 in osteoblasts. MATERIALS AND METHODS: Quantitative reverse-transcriptase polymerase chain reaction (qPCR), RNA interference for netrin receptors, the generation of netrin-1 plasmid, transfection of plasmids, and cell proliferation assay were performed. RESULTS: During osteoblast differentiation by ascorbic acid, netrin-1 expression was significantly decreased. Gene expression related with osteoblast differentiation was down-regulated by netrin-1 treatment. We also found that osteoblast differentiation by bone morphogenetic protein-4 (BMP-4) was inhibited in the presence of recombinant netrin-1. Forced expression of both BMP-4 and netrin-1 significantly decreased alkaline phosphatase expression. On the other hand, Unc5b, neogenin, and A2b which belong to netrin receptors were expressed by osteoblasts. Moreover, alkaline phosphatase expression was significantly decreased by knockdown for the combination of two receptors among these receptors. CONCLUSION: Netrin-1 is involved in the regulation of osteoblast differentiation. |Animals [MESH] |Bone Morphogenetic Protein 4/metabolism [MESH] |Cell Differentiation/*physiology [MESH] |Cell Line [MESH] |Down-Regulation/physiology [MESH] |Gene Expression/physiology [MESH] |Mice [MESH] |Netrin Receptors/metabolism [MESH] |Netrin-1/*metabolism [MESH] |Osteoblasts/*metabolism/*physiology [MESH]Functional Roles of Netrin-1 in Osteoblast Differentiation (epmc).pdf DeepDyve Pubget Overpricing