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2017 ; 49
(3
): e305
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From rabbit antibody repertoires to rabbit monoclonal antibodies
#MMPMID28336958
Weber J
; Peng H
; Rader C
Exp Mol Med
2017[Mar]; 49
(3
): e305
PMID28336958
show ga
In this review, we explain why and how rabbit monoclonal antibodies have become
outstanding reagents for laboratory research and increasingly for diagnostic and
therapeutic applications. Starting with the unique ontogeny of rabbit B cells
that affords highly distinctive antibody repertoires rich in in vivo pruned
binders of high diversity, affinity and specificity, we describe the generation
of rabbit monoclonal antibodies by hybridoma technology, phage display and
alternative methods, along with an account of successful humanization strategies.