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10.1100/tsw.2002.283 http://scihub22266oqcxt.onion/10.1100/tsw.2002.283 C6009261!6009261 !12805918     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\12805918 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       ScientificWorldJournal 2002 ; 2 (ä): 1347-50 Nephropedia Template TP {{tp|p=12805918 |t=2002. Fractionation of hepatic nonparenchymal cells |pdf=|usr=}}{{12805918 }} gab.com Text Fractionation of hepatic nonparenchymal cells JO: ScientificWorldJournal http://www.kidney.de/pget.php?&tw=1&pmid=12805918 #FOAvip The majority of parenchymal cells from mammalian liver cells can be removed by very low speed centrifugation (50 g) but a simple low-density barrier (1.096 g/ml) is required to remove the remaining parenchymal cells from the 50-g supernatant which contains all of the lower density nonparenchymal cells. Continuous gradients of Nycodenz can provide satisfactory resolution of Kupffer, stellate, and endothelial cells on an analytical basis but the separation of different cell types is not sufficient preparatively. Flotation through a low-density iodixanol barrier can, however, provide a satisfactory enrichment of the least dense nonparenchymal cell--the stellate cells. Twit Text FOAVip Fractionation of hepatic nonparenchymal cells ????ScientificWorldJournal http://www.kidney.de/pget.php?&tw=1&pmid=12805918 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=12805918 #FOAvip English Wikipedia <ref>{{Cite pmid|12805918 }}</ref> Fractionation of hepatic nonparenchymal cells #MMPMID12805918 Graham JM ScientificWorldJournal 2002[May]; 2 (ä): 1347-50 PMID12805918 show ga The majority of parenchymal cells from mammalian liver cells can be removed by very low speed centrifugation (50 g) but a simple low-density barrier (1.096 g/ml) is required to remove the remaining parenchymal cells from the 50-g supernatant which contains all of the lower density nonparenchymal cells. Continuous gradients of Nycodenz can provide satisfactory resolution of Kupffer, stellate, and endothelial cells on an analytical basis but the separation of different cell types is not sufficient preparatively. Flotation through a low-density iodixanol barrier can, however, provide a satisfactory enrichment of the least dense nonparenchymal cell--the stellate cells. |Cell Separation/instrumentation/*methods [MESH] |Centrifugation, Density Gradient/instrumentation/*methods [MESH] |Endothelial Cells/cytology [MESH] |Kupffer Cells/cytology [MESH] |Liver/*cytology [MESH] |Solutions [MESH]Fractionation of hepatic nonparenchymal cells (epmc).pdf DeepDyve Pubget Overpricing