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2018 ; 8
(1
): 7968
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Focal brain lesions induced with ultraviolet irradiation
#MMPMID29789523
Nakata M
; Nagasaka K
; Shimoda M
; Takashima I
; Yamamoto S
Sci Rep
2018[May]; 8
(1
): 7968
PMID29789523
show ga
Lesion and inactivation methods have played important roles in neuroscience
studies. However, traditional techniques for creating a brain lesion are highly
invasive, and control of lesion size and shape using these techniques is not
easy. Here, we developed a novel method for creating a lesion on the cortical
surface via 365?nm ultraviolet (UV) irradiation without breaking the dura mater.
We demonstrated that 2.0?mWh UV irradiation, but not the same amount of non-UV
light irradiation, induced an inverted bell-shaped lesion with neuronal loss and
accumulation of glial cells. Moreover, the volume of the UV irradiation-induced
lesion depended on the UV light exposure amount. We further succeeded in
visualizing the lesioned site in a living animal using magnetic resonance imaging
(MRI). Importantly, we also observed using an optical imaging technique that the
spread of neural activation evoked by adjacent cortical stimulation disappeared
only at the UV-irradiated site. In summary, UV irradiation can induce a focal
brain lesion with a stable shape and size in a less invasive manner than
traditional lesioning methods. This method is applicable to not only
neuroscientific lesion experiments but also studies of the focal brain injury
recovery process.
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