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10.1039/c4cp02489c http://scihub22266oqcxt.onion/10.1039/c4cp02489c C4154966!4154966 !25088495     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25088495 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Phys+Chem+Chem+Phys 2014 ; 16 (35 ): 18644-57 Nephropedia Template TP {{tp|p=25088495 |t=2014. Fast single-molecule FRET spectroscopy: theory and experiment |pdf=|usr=}}{{25088495 }} gab.com Text Fast single-molecule FRET spectroscopy: theory and experiment JO: Phys Chem Chem Phys http://www.kidney.de/pget.php?&tw=1&pmid=25088495 #FOAvip Single-molecule spectroscopy is widely used to study macromolecular dynamics. Although this technique provides unique information that cannot be obtained at the ensemble level, the possibility of studying fast molecular dynamics is limited by the number of photons detected per unit time (photon count rate), which is proportional to the illumination intensity. However, simply increasing the illumination intensity often does not help because of various photophysical and photochemical problems. In this Perspective, we show how to improve the dynamic range of single-molecule fluorescence spectroscopy at a given photon count rate by considering each and every photon and using a maximum likelihood method. For a photon trajectory with recorded photon colors and inter-photon times, the parameters of a model describing molecular dynamics are obtained by maximizing the appropriate likelihood function. We discuss various likelihood functions, their applicability, and the accuracy of the extracted parameters. The maximum likelihood method has been applied to analyze the experiments on fast two-state protein folding and to measure transition path times. Utilizing other information such as fluorescence lifetimes is discussed in the framework of two-dimensional FRET efficiency-lifetime histograms. Twit Text FOAVip Fast single-molecule FRET spectroscopy: theory and experiment ????Phys Chem Chem Phys http://www.kidney.de/pget.php?&tw=1&pmid=25088495 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25088495 #FOAvip English Wikipedia <ref>{{Cite pmid|25088495 }}</ref> Fast single-molecule FRET spectroscopy: theory and experiment #MMPMID25088495 Chung HS ; Gopich IV Phys Chem Chem Phys 2014[Sep]; 16 (35 ): 18644-57 PMID25088495 show ga Single-molecule spectroscopy is widely used to study macromolecular dynamics. Although this technique provides unique information that cannot be obtained at the ensemble level, the possibility of studying fast molecular dynamics is limited by the number of photons detected per unit time (photon count rate), which is proportional to the illumination intensity. However, simply increasing the illumination intensity often does not help because of various photophysical and photochemical problems. In this Perspective, we show how to improve the dynamic range of single-molecule fluorescence spectroscopy at a given photon count rate by considering each and every photon and using a maximum likelihood method. For a photon trajectory with recorded photon colors and inter-photon times, the parameters of a model describing molecular dynamics are obtained by maximizing the appropriate likelihood function. We discuss various likelihood functions, their applicability, and the accuracy of the extracted parameters. The maximum likelihood method has been applied to analyze the experiments on fast two-state protein folding and to measure transition path times. Utilizing other information such as fluorescence lifetimes is discussed in the framework of two-dimensional FRET efficiency-lifetime histograms. |*Fluorescence Resonance Energy Transfer [MESH] |*Models, Theoretical [MESH] |Kinetics [MESH] |Molecular Dynamics Simulation [MESH] |Photons [MESH] |Protein Folding [MESH] |Protein Structure, Tertiary [MESH] |Protein Unfolding [MESH]Fast single-molecule FRET spectroscopy: theory and experiment (epmc).pdf DeepDyve Pubget Overpricing