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2016 ; 126
(4
): 1152-62
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Extracellular vesicle isolation and characterization: toward clinical
application
#MMPMID27035807
Xu R
; Greening DW
; Zhu HJ
; Takahashi N
; Simpson RJ
J Clin Invest
2016[Apr]; 126
(4
): 1152-62
PMID27035807
show ga
Two broad categories of extracellular vesicles (EVs), exosomes and shed
microvesicles (sMVs), which differ in size distribution as well as protein and
RNA profiles, have been described. EVs are known to play key roles in cell-cell
communication, acting proximally as well as systemically. This Review discusses
the nature of EV subtypes, strategies for isolating EVs from both cell-culture
media and body fluids, and procedures for quantifying EVs. We also discuss
proteins selectively enriched in exosomes and sMVs that have the potential for
use as markers to discriminate between EV subtypes, as well as various
applications of EVs in clinical diagnosis.