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2014 ; 55
(3
): 1607-15
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Expression of neonatal Fc receptor in the eye
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Powner MB
; McKenzie JA
; Christianson GJ
; Roopenian DC
; Fruttiger M
Invest Ophthalmol Vis Sci
2014[Mar]; 55
(3
): 1607-15
PMID24550358
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PURPOSE: The neonatal Fc receptor (FcRn) plays a critical role in the homeostasis
and degradation of immunoglobulin G (IgG). It mediates the transport of IgG
across epithelial cell barriers and recycles IgG in endothelial cells back into
the bloodstream. These functions critically depend on the binding of FcRn to the
Fc domain of IgG. The half-life and distribution of intravitreally injected
anti-VEGF molecules containing IgG-Fc domains might therefore be affected by FcRn
expressed in the eye. In order to establish whether FcRn-Fc(IgG) interactions may
occur in the eye, we studied the mRNA and protein distribution of FcRn in
postmortem ocular tissue. METHODS: We used qPCR to study mRNA expression of the
transmembrane chain of FcRn (FCGRT) in retina, optic nerve, RPE/choroid plexus,
ciliary body/iris plexus, lens, cornea, and conjunctiva isolated from mouse, rat,
pig, and human postmortem eyes and used immunohistochemistry to determine the
pattern of FcRn expression in FCGRT-transgenic mouse and human eyes. RESULTS: In
all four tested species, Fcgrt mRNA was expressed in the retina, RPE/choroid, and
the ciliary body/iris, while immunohistochemistry documented FcRn protein
expression in the ciliary body epithelium, macrophages, and endothelial cells in
the retinal and choroidal vasculature. CONCLUSIONS: Our results demonstrate that
FcRn has the potential to interact with IgG-Fc domains in the ciliary epithelium
and retinal and choroidal vasculature, which might affect the half-life and
distribution of intravitreally injected Fc-carrying molecules.
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