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2013 ; 29
(12
): 1565-7
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Exome-based analysis for RNA epigenome sequencing data
#MMPMID23589649
Meng J
; Cui X
; Rao MK
; Chen Y
; Huang Y
Bioinformatics
2013[Jun]; 29
(12
): 1565-7
PMID23589649
show ga
MOTIVATION: Fragmented RNA immunoprecipitation combined with RNA sequencing
enabled the unbiased study of RNA epigenome at a near single-base resolution;
however, unique features of this new type of data call for novel computational
techniques. RESULT: Through examining the connections of RNA epigenome sequencing
data with two well-studied data types, ChIP-Seq and RNA-Seq, we unveiled the
salient characteristics of this new data type. The computational strategies were
discussed accordingly, and a novel data processing pipeline was proposed that
combines several existing tools with a newly developed exome-based approach
'exomePeak' for detecting, representing and visualizing the post-transcriptional
RNA modification sites on the transcriptome. AVAILABILITY: The MATLAB package
'exomePeak' and additional details are available at
http://compgenomics.utsa.edu/exomePeak/.