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10.1002/bit.26434 http://scihub22266oqcxt.onion/10.1002/bit.26434 C5698719!5698719 !28842980     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28842980 &cmd=llinks): Failed to open stream: HTTP request failed! 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Escherichia coli "TatExpress" strains super-secrete human growth hormone into the bacterial periplasm by the Tat pathway |pdf=|usr=}}{{28842980 }} gab.com Text Escherichia coli "TatExpress" strains super-secrete human growth hormone into the bacterial periplasm by the Tat pathway JO: Biotechnol Bioeng http://www.kidney.de/pget.php?&tw=1&pmid=28842980 #FOAvip Numerous high-value proteins are secreted into the Escherichia coli periplasm by the General Secretory (Sec) pathway, but Sec-based production chassis cannot handle many potential target proteins. The Tat pathway offers a promising alternative because it transports fully folded proteins; however, yields have been too low for commercial use. To facilitate Tat export, we have engineered the TatExpress series of super-secreting strains by introducing the strong inducible bacterial promoter, ptac, upstream of the chromosomal tatABCD operon, to drive its expression in E. coli strains commonly used by industry (e.g., W3110 and BL21). This modification significantly improves the Tat-dependent secretion of human growth hormone (hGH) into the bacterial periplasm, to the extent that secreted hGH is the dominant periplasmic protein after only 1?hr induction. TatExpress strains accumulate in excess of 30?mg?L(-1) periplasmic recombinant hGH, even in shake flask cultures. A second target protein, an scFv, is also shown to be exported at much higher rates in TatExpress strains. Twit Text FOAVip Escherichia coli "TatExpress" strains super-secrete human growth hormone into the bacterial periplasm by the Tat pathway ????Biotechnol Bioeng http://www.kidney.de/pget.php?&tw=1&pmid=28842980 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28842980 #FOAvip English Wikipedia <ref>{{Cite pmid|28842980 }}</ref> Escherichia coli "TatExpress" strains super-secrete human growth hormone into the bacterial periplasm by the Tat pathway #MMPMID28842980 Browning DF ; Richards KL ; Peswani AR ; Roobol J ; Busby SJW ; Robinson C Biotechnol Bioeng 2017[Dec]; 114 (12 ): 2828-2836 PMID28842980 show ga Numerous high-value proteins are secreted into the Escherichia coli periplasm by the General Secretory (Sec) pathway, but Sec-based production chassis cannot handle many potential target proteins. The Tat pathway offers a promising alternative because it transports fully folded proteins; however, yields have been too low for commercial use. To facilitate Tat export, we have engineered the TatExpress series of super-secreting strains by introducing the strong inducible bacterial promoter, ptac, upstream of the chromosomal tatABCD operon, to drive its expression in E. coli strains commonly used by industry (e.g., W3110 and BL21). This modification significantly improves the Tat-dependent secretion of human growth hormone (hGH) into the bacterial periplasm, to the extent that secreted hGH is the dominant periplasmic protein after only 1?hr induction. TatExpress strains accumulate in excess of 30?mg?L(-1) periplasmic recombinant hGH, even in shake flask cultures. A second target protein, an scFv, is also shown to be exported at much higher rates in TatExpress strains. |Escherichia coli/*genetics/*metabolism [MESH] |Gene Products, tat/*genetics [MESH] |Genetic Enhancement/*methods [MESH] |Growth Hormone/*biosynthesis/genetics/isolation & purification [MESH] |Humans [MESH] |Metabolic Networks and Pathways/genetics [MESH] |Periplasm/*metabolism [MESH] |Promoter Regions, Genetic/genetics [MESH] |Recombinant Proteins/biosynthesis/genetics/isolation & purification [MESH]Escherichia coli "TatExpress" strains super-secrete human growth hormo(epmc).pdf DeepDyve Pubget Overpricing