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10.1038/srep17722 http://scihub22266oqcxt.onion/10.1038/srep17722 C4669507!4669507 !26634810     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26634810 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26634810 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2015 ; 5 (ä): 17722 Nephropedia Template TP {{tp|p=26634810 |t=2015. Enzyme-guided DNA Sewing Architecture |pdf=|usr=}}{{26634810 }} gab.com Text Enzyme-guided DNA Sewing Architecture JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26634810 #FOAvip With the advent of nanotechnology, a variety of nanoarchitectures with varied physicochemical properties have been designed. Owing to the unique characteristics, DNAs have been used as a functional building block for novel nanoarchitecture. In particular, a self-assembly of long DNA molecules via a piece DNA staple has been utilized to attain such constructs. However, it needs many talented prerequisites (e.g., complicated computer program) with fewer yields of products. In addition, it has many limitations to overcome: for instance, (i) thermal instability under moderate environments and (ii) restraint in size caused by the restricted length of scaffold strands. Alternatively, the enzymatic sewing linkage of short DNA blocks is simply designed into long DNA assemblies but it is more error-prone due to the undeveloped sequence data. Here, we present, for the first time, a comprehensive study for directly combining DNA structures into higher DNA sewing constructs through the 5'-end cohesive ligation of T4 enzyme. Inspired by these achievements, the synthesized DNA nanomaterials were also utilized for effective detection and real-time diagnosis of cancer-specific and cytosolic RNA markers. This generalized protocol for generic DNA sewing is expected to be useful in several DNA nanotechnology as well as any nucleic acid-related fields. Twit Text FOAVip Enzyme-guided DNA Sewing Architecture ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26634810 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26634810 #FOAvip English Wikipedia <ref>{{Cite pmid|26634810 }}</ref> Enzyme-guided DNA Sewing Architecture #MMPMID26634810 Song IH ; Shin SW ; Park KS ; Lansac Y ; Jang YH ; Um SH Sci Rep 2015[Dec]; 5 (ä): 17722 PMID26634810 show ga With the advent of nanotechnology, a variety of nanoarchitectures with varied physicochemical properties have been designed. Owing to the unique characteristics, DNAs have been used as a functional building block for novel nanoarchitecture. In particular, a self-assembly of long DNA molecules via a piece DNA staple has been utilized to attain such constructs. However, it needs many talented prerequisites (e.g., complicated computer program) with fewer yields of products. In addition, it has many limitations to overcome: for instance, (i) thermal instability under moderate environments and (ii) restraint in size caused by the restricted length of scaffold strands. Alternatively, the enzymatic sewing linkage of short DNA blocks is simply designed into long DNA assemblies but it is more error-prone due to the undeveloped sequence data. Here, we present, for the first time, a comprehensive study for directly combining DNA structures into higher DNA sewing constructs through the 5'-end cohesive ligation of T4 enzyme. Inspired by these achievements, the synthesized DNA nanomaterials were also utilized for effective detection and real-time diagnosis of cancer-specific and cytosolic RNA markers. This generalized protocol for generic DNA sewing is expected to be useful in several DNA nanotechnology as well as any nucleic acid-related fields. |*Biosensing Techniques [MESH] |*Nanotechnology [MESH] |DNA Ligases/chemistry [MESH] |DNA/*chemistry/genetics [MESH] |Humans [MESH] |Nanostructures/*chemistry [MESH] |Nucleic Acid Conformation [MESH]Enzyme-guided DNA Sewing Architecture (epmc).pdf DeepDyve Pubget Overpricing