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10.1002/anie.201408747 http://scihub22266oqcxt.onion/10.1002/anie.201408747 C4319565!4319565 !25339581     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25339581 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Angew+Chem+Int+Ed+Engl 2014 ; 53 (49 ): 13514-7 Nephropedia Template TP {{tp|p=25339581 |t=2014. Enzymatic fabrication of high-density RNA arrays |pdf=|usr=}}{{25339581 }} gab.com Text Enzymatic fabrication of high-density RNA arrays JO: Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=25339581 #FOAvip A powerful new strategy for the fabrication of high-density RNA arrays is described. A high-density DNA array is fabricated by standard photolithographic methods, the surface-bound DNA molecules are enzymatically copied into their RNA complements from a surface-bound RNA primer, and the DNA templates are enzymatically destroyed, leaving behind the desired RNA array. The strategy is compatible with 2'-fluoro-modified (2'F) ribonucleoside triphosphates (rNTPs), which may be included in the polymerase extension reaction to impart nuclease resistance and other desirable characteristics to the synthesized RNAs. The use and fidelity of the arrays are explored with DNA hybridization, DNAzyme cleavage, and nuclease digestion experiments. Twit Text FOAVip Enzymatic fabrication of high-density RNA arrays ????Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=25339581 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25339581 #FOAvip English Wikipedia <ref>{{Cite pmid|25339581 }}</ref> Enzymatic fabrication of high-density RNA arrays #MMPMID25339581 Wu CH ; Holden MT ; Smith LM Angew Chem Int Ed Engl 2014[Dec]; 53 (49 ): 13514-7 PMID25339581 show ga A powerful new strategy for the fabrication of high-density RNA arrays is described. A high-density DNA array is fabricated by standard photolithographic methods, the surface-bound DNA molecules are enzymatically copied into their RNA complements from a surface-bound RNA primer, and the DNA templates are enzymatically destroyed, leaving behind the desired RNA array. The strategy is compatible with 2'-fluoro-modified (2'F) ribonucleoside triphosphates (rNTPs), which may be included in the polymerase extension reaction to impart nuclease resistance and other desirable characteristics to the synthesized RNAs. The use and fidelity of the arrays are explored with DNA hybridization, DNAzyme cleavage, and nuclease digestion experiments. |*Oligonucleotide Array Sequence Analysis [MESH] |DNA, Catalytic/metabolism [MESH] |Halogenation [MESH] |Nucleic Acid Hybridization [MESH] |RNA/*chemistry/metabolism [MESH]Enzymatic fabrication of high-density RNA arrays (epmc).pdf DeepDyve Pubget Overpricing