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2014 ; 18
(6
): 962-5
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Enucleation: a possible mechanism of cancer cell death
#MMPMID24629135
Paunescu V
; Bojin FM
; Gavriliuc OI
; Taculescu EA
; Ianos R
; Ordodi VL
; Iman VF
; Tatu CA
J Cell Mol Med
2014[Jun]; 18
(6
): 962-5
PMID24629135
show ga
There are few major morphologies of cell death that have been described so far:
apoptosis (type I), cell death associated with autophagy (type II), necrosis
(type III) and anchorage-dependent mechanisms-anoikis. Here, we show for the
first time a possibly novel mechanism inducing tumour cell death under in vitro
conditions-enucleation. We pursued the influence of colloidal suspensions of Fe3
O4 nanoparticles on tumour cell lines (SK-BR-3 and MCF-7 breast cancer cell
lines) grown according to standard cell culture protocols. Magnetite
nanoparticles were prepared by combustion synthesis and double layer coated with
oleic acid. Scanning and transmission electron microscopy revealed that tumour
cells developed a network of intracytoplasmic stress fibres, which induce
extrusion of nuclei, and enucleated cells die. Normal adult mesenchymal stem
cells, used as control, did not exhibit the same behaviour. Intact nuclei were
found in culture supernatant of tumour cells, and were visualized by
immunofluorescence. Enucleation as a potential mechanism of tumour cell death
might open new horizons in cancer biology research and development of therapeutic
agents capable of exploiting this behaviour.
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