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Engineering of Metabolic Pathways by Artificial Enzyme Channels
#MMPMID26557643
Pröschel M
; Detsch R
; Boccaccini AR
; Sonnewald U
Front Bioeng Biotechnol
2015[]; 3
(?): 168
PMID26557643
show ga
Application of industrial enzymes for production of valuable chemical compounds
has greatly benefited from recent developments in Systems and Synthetic Biology.
Both, in vivo and in vitro systems have been established, allowing conversion of
simple into complex compounds. Metabolic engineering in living cells needs to be
balanced which is achieved by controlling gene expression levels, translation,
scaffolding, compartmentation, and flux control. In vitro applications are often
hampered by limited protein stability/half-life and insufficient rates of
substrate conversion. To improve stability and catalytic activity, proteins are
post-translationally modified and arranged in artificial metabolic channels.
Within the review article, we will first discuss the supramolecular organization
of enzymes in living systems and second summarize current and future approaches
to design artificial metabolic channels by additive manufacturing for the
efficient production of desired products.
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