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2017 ; 7
(ä): 45393
Nephropedia Template TP
Doyle F
; Lapsia S
; Spadaro S
; Wurz ZE
; Bhaduri-McIntosh S
; Tenenbaum SA
Sci Rep
2017[Mar]; 7
(ä): 45393
PMID28350000
show ga
RNA-based three-way junctions (3WJs) are naturally occurring structures found in
many functional RNA molecules including rRNA, tRNA, snRNA and ribozymes. 3WJs are
typically characterized as resulting from an RNA molecule folding back on itself
in cis but could also form in trans when one RNA, for instance a microRNA binds
to a second structured RNA, such as a mRNA. Trans-3WJs can influence the final
shape of one or both of the RNA molecules and can thus provide a means for
modulating the availability of regulatory motifs including potential protein or
microRNA binding sites. Regulatory 3WJs generated in trans represent a newly
identified regulatory category that we call structurally interacting RNA or sxRNA
for convenience. Here we show that they can be rationally designed using familiar
cis-3WJ examples as a guide. We demonstrate that an sxRNA "bait" sequence can be
designed to interact with a specific microRNA "trigger" sequence, creating a
regulatable RNA-binding protein motif that retains its functional activity.
Further, we show that when placed downstream of a coding sequence, sxRNA can be
used to switch "ON" translation of that sequence in the presence of the trigger
microRNA and the amount of translation corresponded with the amount of microRNA
present.
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