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10.1038/mt.2015.164 http://scihub22266oqcxt.onion/10.1038/mt.2015.164 C4786910!4786910 !26336974     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26336974 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26336974 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Ther 2016 ; 24 (3 ): 447-57 Nephropedia Template TP {{tp|p=26336974 |t=2016. Engineered Viruses as Genome Editing Devices |pdf=|usr=}}{{26336974 }} gab.com Text Engineered Viruses as Genome Editing Devices JO: Mol Ther http://www.kidney.de/pget.php?&tw=1&pmid=26336974 #FOAvip Genome editing based on sequence-specific designer nucleases, also known as programmable nucleases, seeks to modify in a targeted and precise manner the genetic information content of living cells. Delivering into cells designer nucleases alone or together with donor DNA templates, which serve as surrogate homologous recombination (HR) substrates, can result in gene knockouts or gene knock-ins, respectively. As engineered replication-defective viruses, viral vectors are having an increasingly important role as delivery vehicles for donor DNA templates and designer nucleases, namely, zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated Cas9 (CRISPR-Cas9) nucleases, also known as RNA-guided nucleases (RGNs). We review this dual role played by engineered viral particles on genome editing while focusing on their main scaffolds, consisting of lentiviruses, adeno-associated viruses, and adenoviruses. In addition, the coverage of the growing body of research on the repurposing of viral vectors as delivery systems for genome editing tools is complemented with information regarding their main characteristics, pros, and cons. Finally, this information is framed by a concise description of the chief principles, tools, and applications of the genome editing field as a whole. Twit Text FOAVip Engineered Viruses as Genome Editing Devices ????Mol Ther http://www.kidney.de/pget.php?&tw=1&pmid=26336974 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26336974 #FOAvip English Wikipedia <ref>{{Cite pmid|26336974 }}</ref> Engineered Viruses as Genome Editing Devices #MMPMID26336974 Chen X ; Gonçalves MA Mol Ther 2016[Mar]; 24 (3 ): 447-57 PMID26336974 show ga Genome editing based on sequence-specific designer nucleases, also known as programmable nucleases, seeks to modify in a targeted and precise manner the genetic information content of living cells. Delivering into cells designer nucleases alone or together with donor DNA templates, which serve as surrogate homologous recombination (HR) substrates, can result in gene knockouts or gene knock-ins, respectively. As engineered replication-defective viruses, viral vectors are having an increasingly important role as delivery vehicles for donor DNA templates and designer nucleases, namely, zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated Cas9 (CRISPR-Cas9) nucleases, also known as RNA-guided nucleases (RGNs). We review this dual role played by engineered viral particles on genome editing while focusing on their main scaffolds, consisting of lentiviruses, adeno-associated viruses, and adenoviruses. In addition, the coverage of the growing body of research on the repurposing of viral vectors as delivery systems for genome editing tools is complemented with information regarding their main characteristics, pros, and cons. Finally, this information is framed by a concise description of the chief principles, tools, and applications of the genome editing field as a whole. |*Gene Editing/methods [MESH] |*Genetic Engineering/methods [MESH] |*Genome [MESH] |Animals [MESH] |Endonucleases/genetics/metabolism [MESH] |Gene Targeting [MESH] |Genetic Vectors/*genetics [MESH] |Humans [MESH]Engineered Viruses as Genome Editing Devices (epmc).pdf DeepDyve Pubget Overpricing