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10.3389/fphys.2017.00275 http://scihub22266oqcxt.onion/10.3389/fphys.2017.00275 C5418228!5418228 !28529488     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28529488 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Front+Physiol 2017 ; 8 (ä): 275 Nephropedia Template TP {{tp|p=28529488 |t=2017. Endothelial Extracellular Vesicles-Promises and Challenges |pdf=|usr=}}{{28529488 }} gab.com Text Endothelial Extracellular Vesicles-Promises and Challenges JO: Front Physiol http://www.kidney.de/pget.php?&tw=1&pmid=28529488 #FOAvip Extracellular vesicles, including exosomes, microparticles, and apoptotic bodies, are phospholipid bilayer-enclosed vesicles that have once been considered as cell debris lacking biological functions. However, they have recently gained immense interest in the scientific community due to their role in intercellular communication, immunity, tissue regeneration as well as in the onset, and progression of various pathologic conditions. Extracellular vesicles of endothelial origin have been found to play a versatile role in the human body, since they are on the one hand known to contribute to cardiovascular diseases, but on the other hand have also been reported to promote endothelial cell survival. Hence, endothelial extracellular vesicles hold promising therapeutic potential to be used as a new tool to detect as well as treat a great number of diseases. This calls for clinically approved, standardized, and efficient isolation and characterization protocols to harvest and purify endothelial extracellular vesicles. However, such methods and techniques to fulfill stringent requirements for clinical trials have yet to be developed or are not harmonized internationally. In this review, recent advances and challenges in the field of endothelial extracellular vesicle research are discussed and current problems and limitations regarding isolation and characterization are pointed out. Twit Text FOAVip Endothelial Extracellular Vesicles-Promises and Challenges ????Front Physiol http://www.kidney.de/pget.php?&tw=1&pmid=28529488 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28529488 #FOAvip English Wikipedia <ref>{{Cite pmid|28529488 }}</ref> Endothelial Extracellular Vesicles-Promises and Challenges #MMPMID28529488 Hromada C ; Mühleder S ; Grillari J ; Redl H ; Holnthoner W Front Physiol 2017[]; 8 (ä): 275 PMID28529488 show ga Extracellular vesicles, including exosomes, microparticles, and apoptotic bodies, are phospholipid bilayer-enclosed vesicles that have once been considered as cell debris lacking biological functions. However, they have recently gained immense interest in the scientific community due to their role in intercellular communication, immunity, tissue regeneration as well as in the onset, and progression of various pathologic conditions. Extracellular vesicles of endothelial origin have been found to play a versatile role in the human body, since they are on the one hand known to contribute to cardiovascular diseases, but on the other hand have also been reported to promote endothelial cell survival. Hence, endothelial extracellular vesicles hold promising therapeutic potential to be used as a new tool to detect as well as treat a great number of diseases. This calls for clinically approved, standardized, and efficient isolation and characterization protocols to harvest and purify endothelial extracellular vesicles. However, such methods and techniques to fulfill stringent requirements for clinical trials have yet to be developed or are not harmonized internationally. In this review, recent advances and challenges in the field of endothelial extracellular vesicle research are discussed and current problems and limitations regarding isolation and characterization are pointed out. äEndothelial Extracellular Vesicles-Promises and Challenges (epmc).pdf DeepDyve Pubget Overpricing