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Elevation of Autoantibody in Patients with Ischemic Stroke
#MMPMID29848906
Yoshida Y
; Hiwasa T
; Machida T
; Kobayashi E
; Mine S
; Matsushima J
; Takiguchi M
; Iwadate Y
Neurol Med Chir (Tokyo)
2018[Jul]; 58
(7
): 303-310
PMID29848906
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Recent clinical research has revealed a significant correlation between
atherosclerosis, one of the primary etiologies of ischemic stroke, and the immune
system. Assuming that "disease-specific autoantibodies are produced in the sera
of patients with ischemic stroke," we investigated multiple
arteriosclerosis-related antibodies using the serological identification of
antigens by recombinant cDNA expression cloning (SEREX), an established method
for identifying antigenic proteins. We either screened a human aortic endothelial
cell cDNA library or conducted protein array screening using the sera from
patients with ischemic stroke, such as carotid artery stenosis or transient
ischemic attack (TIA). Next, we measured serum antibody levels using amplified
luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) in
patient/healthy donor (HD) cohorts and identified several antigens, the antibody
levels of which were significantly higher in patients with ischemic stroke than
in HDs. This review introduced the method of identifying antigens by the SEREX
and protein microarray and summarized antigenic proteins. In particular, it
focused on anti-replication protein A2 antibody and anti-programmed cell death 11
antibody, which are significantly related to atherosclerotic plaque and ischemic
brain tissue, respectively, and proposed the mechanism of elevated autoantibody
levels against them. Furthermore, this review suggests a possibility of clinical
application as an atherosclerotic disease diagnostic marker for TIA or cerebral
infarction.
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