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2016 ; 6
(ä): 253-259
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Electroporation by subnanosecond pulses
#MMPMID27482547
Semenov I
; Xiao S
; Pakhomov AG
Biochem Biophys Rep
2016[Jul]; 6
(ä): 253-259
PMID27482547
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Electropermeabilization of cell membranes by micro- and nanosecond-duration
stimuli has been studied extensively, whereas effects of picosecond electric
pulses (psEP) remain essentially unexplored. We utilized whole-cell patch clamp
and Di-8-ANEPPS voltage-sensitive dye measurements to characterize plasma
membrane effects of 500 ps stimuli in rat hippocampal neurons (RHN), NG108, and
CHO cells. Even a single 500-ps pulse at 190 kV/cm increased membrane conductance
and depolarized cells. These effects were augmented by applying brief psEP bursts
(5-125 pulses), whereas the rate of pulse delivery (8Hz - 1 kHz) played little
role. psEP-treated cells displayed large inward current at negative membrane
potentials but modest or no conductance changes at positive potentials. A 1-kHz
burst of 25 pulses increased the whole-cell conductance in the range (-100) -
(-60) mV to 22-26 nS in RHN and NG108 cells (from 3 and 0.7 nS, respectively),
but only to 5 nS in CHO (from 0.3 nS). The conductance increase was reversible
within about 2 min. Such pattern of cell permeabilization, with characteristic
inward rectification and slow recovery, was similar to earlier reported effects
of 60- and 600-ns pulses, pointing to the similarity of structural membrane
rearrangements in spite of a different membrane charging mechanism.
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