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2015 ; 82
(ä): 10.7.1-10.7.16
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Electroblotting from Polyacrylamide Gels
#MMPMID26521711
Goldman A
; Ursitti JA
; Mozdzanowski J
; Speicher DW
Curr Protoc Protein Sci
2015[Nov]; 82
(ä): 10.7.1-10.7.16
PMID26521711
show ga
Transferring proteins from polyacrylamide gels onto retentive membranes is now
primarily used for immunoblotting. A second application that was quite common up
to about a decade ago was electroblotting of proteins for N-terminal and internal
sequencing using Edman chemistry. This unit contains procedures for
electroblotting proteins from polyacrylamide gels onto a variety of membranes,
including polyvinylidene difluoride (PVDF) and nitrocellulose. In addition to the
commonly used tank or wet transfer system, protocols are provided for
electroblotting using semidry and dry systems. This unit also describes
procedures for eluting proteins from membranes using detergents or acidic
extraction with organic solvents for specialized applications.