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10.1371/journal.pone.0161242 http://scihub22266oqcxt.onion/10.1371/journal.pone.0161242 C5006992!5006992 !27579919     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27579919 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27579919 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       PLoS+One 2016 ; 11 (8 ): e0161242 Nephropedia Template TP {{tp|p=27579919 |t=2016. Effect of Predatory Bacteria on Human Cell Lines |pdf=|usr=}}{{27579919 }} gab.com Text Effect of Predatory Bacteria on Human Cell Lines JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=27579919 #FOAvip Predatory bacteria are Gram-negative bacteria that prey on other Gram-negative bacteria and have been considered as potential therapeutic agents against multi-drug resistant pathogens. In vivo animal models have demonstrated that predatory bacteria are non-toxic and non-immunogenic in rodents. In order to consider the use of predatory bacteria as live antibiotics, it is important to investigate their effect on human cells. The aim of this study was to determine the effect of Bdellovibrio bacteriovorus strains 109J and HD100, and Micavibrio aeruginosavorus strain ARL-13 on cell viability and inflammatory responses of five human cell lines, representative of clinically relevant tissues. We found that the predators were not cytotoxic to any of the human cell lines tested. Microscopic imaging showed no signs of cell detachment, as compared to predator-free cells. In comparison to an E. coli control, exposure to higher concentrations of the predators did not trigger a significant elevation of pro-inflammatory cytokines in four of the five human cell lines tested. Our work underlines the non-pathogenic attributes of predatory bacteria on human cells and highlights their potential use as live antibiotics against human pathogens. Twit Text FOAVip Effect of Predatory Bacteria on Human Cell Lines ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=27579919 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27579919 #FOAvip English Wikipedia <ref>{{Cite pmid|27579919 }}</ref> Effect of Predatory Bacteria on Human Cell Lines #MMPMID27579919 Gupta S ; Tang C ; Tran M ; Kadouri DE PLoS One 2016[]; 11 (8 ): e0161242 PMID27579919 show ga Predatory bacteria are Gram-negative bacteria that prey on other Gram-negative bacteria and have been considered as potential therapeutic agents against multi-drug resistant pathogens. In vivo animal models have demonstrated that predatory bacteria are non-toxic and non-immunogenic in rodents. In order to consider the use of predatory bacteria as live antibiotics, it is important to investigate their effect on human cells. The aim of this study was to determine the effect of Bdellovibrio bacteriovorus strains 109J and HD100, and Micavibrio aeruginosavorus strain ARL-13 on cell viability and inflammatory responses of five human cell lines, representative of clinically relevant tissues. We found that the predators were not cytotoxic to any of the human cell lines tested. Microscopic imaging showed no signs of cell detachment, as compared to predator-free cells. In comparison to an E. coli control, exposure to higher concentrations of the predators did not trigger a significant elevation of pro-inflammatory cytokines in four of the five human cell lines tested. Our work underlines the non-pathogenic attributes of predatory bacteria on human cells and highlights their potential use as live antibiotics against human pathogens. |Bdellovibrio bacteriovorus/*metabolism [MESH] |Cell Line [MESH] |Escherichia coli Infections/*metabolism [MESH] |Escherichia coli/*metabolism [MESH] |Humans [MESH]Effect of Predatory Bacteria on Human Cell Lines (epmc).pdf DeepDyve Pubget Overpricing