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2016 ; 72
(Pt 9
): 1036-48
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EIGER detector: application in macromolecular crystallography
#MMPMID27599736
Casanas A
; Warshamanage R
; Finke AD
; Panepucci E
; Olieric V
; Nöll A
; Tampé R
; Brandstetter S
; Förster A
; Mueller M
; Schulze-Briese C
; Bunk O
; Wang M
Acta Crystallogr D Struct Biol
2016[Sep]; 72
(Pt 9
): 1036-48
PMID27599736
show ga
The development of single-photon-counting detectors, such as the PILATUS, has
been a major recent breakthrough in macromolecular crystallography, enabling
noise-free detection and novel data-acquisition modes. The new EIGER detector
features a pixel size of 75 × 75?µm, frame rates of up to 3000?Hz and a dead time
as low as 3.8?µs. An EIGER 1M and EIGER 16M were tested on Swiss Light Source
beamlines X10SA and X06SA for their application in macromolecular
crystallography. The combination of fast frame rates and a very short dead time
allows high-quality data acquisition in a shorter time. The ultrafine ?-slicing
data-collection method is introduced and validated and its application in finding
the optimal rotation angle, a suitable rotation speed and a sufficient X-ray dose
are presented. An improvement of the data quality up to slicing at one tenth of
the mosaicity has been observed, which is much finer than expected based on
previous findings. The influence of key data-collection parameters on data
quality is discussed.