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2014 ; 107
(12
): L37-L40
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Dynamics of cell area and force during spreading
#MMPMID25517168
Brill-Karniely Y
; Nisenholz N
; Rajendran K
; Dang Q
; Krishnan R
; Zemel A
Biophys J
2014[Dec]; 107
(12
): L37-L40
PMID25517168
show ga
Experiments on human pulmonary artery endothelial cells are presented to show
that cell area and the force exerted on a substrate increase simultaneously, but
with different rates during spreading; rapid-force increase systematically
occurred several minutes past initial spreading. We examine this theoretically
and present three complementary mechanisms that may accompany the development of
lamellar stress during spreading and underlie the observed behavior. These
include: 1), the dynamics of cytoskeleton assembly at the cell basis; 2), the
strengthening of acto-myosin forces in response to the generated lamellar
stresses; and 3), the passive strain-stiffening of the cytoskeleton.