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2015 ; 169
(3
): 1524-31
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Discovering New Biology through Sequencing of RNA
#MMPMID26353759
Weber AP
Plant Physiol
2015[Nov]; 169
(3
): 1524-31
PMID26353759
show ga
Sequencing of RNA (RNA-Seq) was invented approximately 1 decade ago and has since
revolutionized biological research. This update provides a brief historic
perspective on the development of RNA-Seq and then focuses on the application of
RNA-Seq in qualitative and quantitative analyses of transcriptomes. Particular
emphasis is given to aspects of data analysis. Since the wet-lab and data
analysis aspects of RNA-Seq are still rapidly evolving and novel applications are
continuously reported, a printed review will be rapidly outdated and can only
serve to provide some examples and general guidelines for planning and conducting
RNA-Seq studies. Hence, selected references to frequently update online resources
are given.