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10.1002/cpnc.4

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C4905557!4905557 !27248784
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suck abstract from ncbi


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pmid27248784
      Curr+Protoc+Nucleic+Acid+Chem 2016 ; 65 (ä): 7.13.1-7.13.41
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  • Diffraction Techniques in Structural Biology #MMPMID27248784
  • Egli M
  • Curr Protoc Nucleic Acid Chem 2016[Jun]; 65 (ä): 7.13.1-7.13.41 PMID27248784 show ga
  • A detailed understanding of chemical and biological function and the mechanisms underlying the molecular activities ultimately requires atomic-resolution structural data. Diffraction-based techniques such as single-crystal X-ray crystallography, electron microscopy, and neutron diffraction are well established and they have paved the road to the stunning successes of modern-day structural biology. The major advances achieved in the last twenty years in all aspects of structural research, including sample preparation, crystallization, the construction of synchrotron and spallation sources, phasing approaches, and high-speed computing and visualization, now provide specialists and nonspecialists alike with a steady flow of molecular images of unprecedented detail. The present unit combines a general overview of diffraction methods with a detailed description of the process of a single-crystal X-ray structure determination experiment, from chemical synthesis or expression to phasing and refinement, analysis, and quality control. For novices it may serve as a stepping-stone to more in-depth treatises of the individual topics. Readers relying on structural information for interpreting functional data may find it a useful consumer guide. © 2016 by John Wiley & Sons, Inc.
  • |Crystallography, X-Ray/methods [MESH]
  • |Magnetic Resonance Spectroscopy/*methods [MESH]
  • |Microscopy, Electron/methods [MESH]
  • |Molecular Biology/*methods [MESH]
  • |Neutron Diffraction/methods [MESH]


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