10.1002/cpnc.4 http://scihub22266oqcxt.onion/10.1002/cpnc.4 C4905557!4905557
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Curr+Protoc+Nucleic+Acid+Chem
2016 ; 65
(ä): 7.13.1-7.13.41
Nephropedia Template TP gab.com Text Twit Text FOAVip Twit Text # English Wikipedia
Diffraction Techniques in Structural Biology
#MMPMID27248784
Egli M
Curr Protoc Nucleic Acid Chem
2016[Jun]; 65
(ä): 7.13.1-7.13.41
PMID27248784
show ga
A detailed understanding of chemical and biological function and the mechanisms
underlying the molecular activities ultimately requires atomic-resolution
structural data. Diffraction-based techniques such as single-crystal X-ray
crystallography, electron microscopy, and neutron diffraction are well
established and they have paved the road to the stunning successes of modern-day
structural biology. The major advances achieved in the last twenty years in all
aspects of structural research, including sample preparation, crystallization,
the construction of synchrotron and spallation sources, phasing approaches, and
high-speed computing and visualization, now provide specialists and
nonspecialists alike with a steady flow of molecular images of unprecedented
detail. The present unit combines a general overview of diffraction methods with
a detailed description of the process of a single-crystal X-ray structure
determination experiment, from chemical synthesis or expression to phasing and
refinement, analysis, and quality control. For novices it may serve as a
stepping-stone to more in-depth treatises of the individual topics. Readers
relying on structural information for interpreting functional data may find it a
useful consumer guide. © 2016 by John Wiley & Sons, Inc.
Please enable JavaScript to view the comments powered by Disqus. |Crystallography, X-Ray/methods
[MESH] |Magnetic Resonance Spectroscopy/*methods
[MESH] |Microscopy, Electron/methods
[MESH] |Molecular Biology/*methods
[MESH] |Neutron Diffraction/methods
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