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10.1038/ncomms14853 http://scihub22266oqcxt.onion/10.1038/ncomms14853 C5394234!5394234 !28393831     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28393831 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Commun 2017 ; 8 (ä): 14853 Nephropedia Template TP {{tp|p=28393831 |t=2017. Determining the bacterial cell biology of Planctomycetes |pdf=|usr=}}{{28393831 }} gab.com Text Determining the bacterial cell biology of Planctomycetes JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28393831 #FOAvip Bacteria of the phylum Planctomycetes have been previously reported to possess several features that are typical of eukaryotes, such as cytosolic compartmentalization and endocytosis-like macromolecule uptake. However, recent evidence points towards a Gram-negative cell plan for Planctomycetes, although in-depth experimental analysis has been hampered by insufficient genetic tools. Here we develop methods for expression of fluorescent proteins and for gene deletion in a model planctomycete, Planctopirus limnophila, to analyse its cell organization in detail. Super-resolution light microscopy of mutants, cryo-electron tomography, bioinformatic predictions and proteomic analyses support an altered Gram-negative cell plan for Planctomycetes, including a defined outer membrane, a periplasmic space that can be greatly enlarged and convoluted, and an energized cytoplasmic membrane. These conclusions are further supported by experiments performed with two other Planctomycetes, Gemmata obscuriglobus and Rhodopirellula baltica. We also provide experimental evidence that is inconsistent with endocytosis-like macromolecule uptake; instead, extracellular macromolecules can be taken up and accumulate in the periplasmic space through unclear mechanisms. Twit Text FOAVip Determining the bacterial cell biology of Planctomycetes ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28393831 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28393831 #FOAvip English Wikipedia <ref>{{Cite pmid|28393831 }}</ref> Determining the bacterial cell biology of Planctomycetes #MMPMID28393831 Boedeker C ; Schüler M ; Reintjes G ; Jeske O ; van Teeseling MC ; Jogler M ; Rast P ; Borchert D ; Devos DP ; Kucklick M ; Schaffer M ; Kolter R ; van Niftrik L ; Engelmann S ; Amann R ; Rohde M ; Engelhardt H ; Jogler C Nat Commun 2017[Apr]; 8 (ä): 14853 PMID28393831 show ga Bacteria of the phylum Planctomycetes have been previously reported to possess several features that are typical of eukaryotes, such as cytosolic compartmentalization and endocytosis-like macromolecule uptake. However, recent evidence points towards a Gram-negative cell plan for Planctomycetes, although in-depth experimental analysis has been hampered by insufficient genetic tools. Here we develop methods for expression of fluorescent proteins and for gene deletion in a model planctomycete, Planctopirus limnophila, to analyse its cell organization in detail. Super-resolution light microscopy of mutants, cryo-electron tomography, bioinformatic predictions and proteomic analyses support an altered Gram-negative cell plan for Planctomycetes, including a defined outer membrane, a periplasmic space that can be greatly enlarged and convoluted, and an energized cytoplasmic membrane. These conclusions are further supported by experiments performed with two other Planctomycetes, Gemmata obscuriglobus and Rhodopirellula baltica. We also provide experimental evidence that is inconsistent with endocytosis-like macromolecule uptake; instead, extracellular macromolecules can be taken up and accumulate in the periplasmic space through unclear mechanisms. |Ammonia/metabolism [MESH] |Endocytosis [MESH] |Genomics [MESH] |Oxidation-Reduction [MESH] |Phylogeny [MESH] |Planctomycetales/classification/genetics/*metabolism/physiology [MESH]Determining the bacterial cell biology of Planctomycetes (epmc).pdf DeepDyve Pubget Overpricing