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Detection of Sphingomyelin Clusters by Raman Spectroscopy
#MMPMID27602727
Shirota K
; Yagi K
; Inaba T
; Li PC
; Murata M
; Sugita Y
; Kobayashi T
Biophys J
2016[Sep]; 111
(5
): 999-1007
PMID27602727
show ga
Sphingomyelin (SM) is a major sphingolipid in mammalian cells that forms specific
lipid domains in combination with cholesterol (Chol). Using molecular-dynamics
simulation and density functional theory calculation, we identified a
characteristic Raman band of SM at ?1643 cm(-1) as amide I of the SM cluster.
Experimental results indicate that this band is sensitive to the hydration of SM
and the presence of Chol. We showed that this amide I Raman band can be utilized
to examine the membrane distribution of SM. Similarly to SM, ceramide
phosphoethanolamine (CerPE) exhibited an amide I Raman band in almost the same
region, although CerPE lacks three methyl groups in the phosphocholine moiety of
SM. In contrast to SM, the amide I band of CerPE was not affected by Chol,
suggesting the importance of the methyl groups of SM in the SM-Chol interaction.
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