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2015 ; 93
(3
): 475-7
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Detection of Entebbe Bat Virus After 54 Years
#MMPMID26101270
Kading RC
; Kityo R
; Nakayiki T
; Ledermann J
; Crabtree MB
; Lutwama J
; Miller BR
Am J Trop Med Hyg
2015[Sep]; 93
(3
): 475-7
PMID26101270
show ga
Entebbe bat virus (ENTV; Flaviviridae: Flavivirus), closely related to yellow
fever virus, was first isolated from a little free-tailed bat (Chaerephon
pumilus) in Uganda in 1957, but was not detected after that initial isolation. In
2011, we isolated ENTV from a little free-tailed bat captured from the attic of a
house near where it had originally been found. Infectious virus was recovered
from the spleen and lung, and the viral RNA was sequenced and compared with that
of the original isolate. Across the polypeptide sequence, there were 76 amino
acid substitutions, resulting in 97.8% identity at the amino acid level between
the 1957 and 2011 isolates. Further study of this virus would provide valuable
insights into the ecological and genetic factors governing the evolution and
transmission of bat- and mosquito-borne flaviviruses.