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10.1007/978-1-4939-3299-3_5 http://scihub22266oqcxt.onion/10.1007/978-1-4939-3299-3_5 C4857604!4857604 !27033070     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27033070 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Methods+Mol+Biol 2016 ; 1388 (ä): 53-62 Nephropedia Template TP {{tp|p=27033070 |t=2016. Detecting PTEN and PI3K Signaling in Brain |pdf=|usr=}}{{27033070 }} gab.com Text Detecting PTEN and PI3K Signaling in Brain JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=27033070 #FOAvip The central nervous system is comprised of multiple cell types including neurons, glia, and other supporting cells that may differ dramatically in levels of signaling pathway activation. Immunohistochemistry in conjunction with drug interference are powerful tools that allow evaluation of signaling pathways in different cell types of the mouse central nervous system in vivo. Here we provide detailed protocols for immunohistochemistry to evaluate three essential components in the PI3K pathway in mouse brain: Pten, p-Akt, and p-4ebp1, and for rapamycin treatment to modulate mTOR signaling in vivo. Twit Text FOAVip Detecting PTEN and PI3K Signaling in Brain ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=27033070 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27033070 #FOAvip English Wikipedia <ref>{{Cite pmid|27033070 }}</ref> Detecting PTEN and PI3K Signaling in Brain #MMPMID27033070 Zhu G ; Baker SJ Methods Mol Biol 2016[]; 1388 (ä): 53-62 PMID27033070 show ga The central nervous system is comprised of multiple cell types including neurons, glia, and other supporting cells that may differ dramatically in levels of signaling pathway activation. Immunohistochemistry in conjunction with drug interference are powerful tools that allow evaluation of signaling pathways in different cell types of the mouse central nervous system in vivo. Here we provide detailed protocols for immunohistochemistry to evaluate three essential components in the PI3K pathway in mouse brain: Pten, p-Akt, and p-4ebp1, and for rapamycin treatment to modulate mTOR signaling in vivo. |*Signal Transduction [MESH] |Animals [MESH] |Brain/*metabolism [MESH] |Immunohistochemistry/*methods [MESH] |Mice [MESH] |PTEN Phosphohydrolase/*metabolism [MESH] |Phosphatidylinositol 3-Kinases/metabolism [MESH]Detecting PTEN and PI3K Signaling in Brain (epmc).pdf DeepDyve Pubget Overpricing