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10.1016/j.ymeth.2015.05.022

http://scihub22266oqcxt.onion/10.1016/j.ymeth.2015.05.022
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suck abstract from ncbi


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pmid26032817
      Methods 2015 ; 86 (ä): 80-8
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  • Defining bacterial regulons using ChIP-seq #MMPMID26032817
  • Myers KS ; Park DM ; Beauchene NA ; Kiley PJ
  • Methods 2015[Sep]; 86 (ä): 80-8 PMID26032817 show ga
  • Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) is a powerful method that identifies protein-DNA binding sites in vivo. Recent studies have illustrated the value of ChIP-seq in studying transcription factor binding in various bacterial species under a variety of growth conditions. These results show that in addition to identifying binding sites, correlation of ChIP-seq data with expression data can reveal important information about bacterial regulons and regulatory networks. In this chapter, we provide an overview of the current state of knowledge about ChIP-seq methodology in bacteria, from sample preparation to raw data analysis. We also describe visualization and various bioinformatic analyses of processed ChIP-seq data.
  • |Bacteria/genetics [MESH]
  • |Binding Sites [MESH]
  • |Computational Biology/methods [MESH]
  • |DNA-Binding Proteins/chemistry/*genetics [MESH]
  • |High-Throughput Nucleotide Sequencing/*methods [MESH]
  • |Molecular Biology/*methods [MESH]
  • |Oligonucleotide Array Sequence Analysis/methods [MESH]
  • |Regulon/*genetics [MESH]


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