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2015 ; 112
(15
): 4564-9
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DNA-mediated engineering of multicomponent enzyme crystals
#MMPMID25831510
Brodin JD
; Auyeung E
; Mirkin CA
Proc Natl Acad Sci U S A
2015[Apr]; 112
(15
): 4564-9
PMID25831510
show ga
The ability to predictably control the coassembly of multiple nanoscale building
blocks, especially those with disparate chemical and physical properties such as
biomolecules and inorganic nanoparticles, has far-reaching implications in
catalysis, sensing, and photonics, but a generalizable strategy for engineering
specific contacts between these particles is an outstanding challenge. This is
especially true in the case of proteins, where the types of possible
interparticle interactions are numerous, diverse, and complex. Herein, we explore
the concept of trading protein-protein interactions for DNA-DNA interactions to
direct the assembly of two nucleic-acid-functionalized proteins with distinct
surface chemistries into six unique lattices composed of catalytically active
proteins, or of a combination of proteins and DNA-modified gold nanoparticles.
The programmable nature of DNA-DNA interactions used in this strategy allows us
to control the lattice symmetries and unit cell constants, as well as the
compositions and habit, of the resulting crystals. This study provides a
potentially generalizable strategy for constructing a unique class of materials
that take advantage of the diverse morphologies, surface chemistries, and
functionalities of proteins for assembling functional crystalline materials.
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