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2016 ; 52
(18
): 3598-610
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DNA-based control of protein activity
#MMPMID26812623
Engelen W
; Janssen BM
; Merkx M
Chem Commun (Camb)
2016[Mar]; 52
(18
): 3598-610
PMID26812623
show ga
DNA has emerged as a highly versatile construction material for nanometer-sized
structures and sophisticated molecular machines and circuits. The successful
application of nucleic acid based systems greatly relies on their ability to
autonomously sense and act on their environment. In this feature article, the
development of DNA-based strategies to dynamically control protein activity via
oligonucleotide triggers is discussed. Depending on the desired application,
protein activity can be controlled by directly conjugating them to an
oligonucleotide handle, or expressing them as a fusion protein with DNA binding
motifs. To control proteins without modifying them chemically or genetically,
multivalent ligands and aptamers that reversibly inhibit their function provide
valuable tools to regulate proteins in a noncovalent manner. The goal of this
feature article is to give an overview of strategies developed to control protein
activity via oligonucleotide-based triggers, as well as hurdles yet to be taken
to obtain fully autonomous systems that interrogate, process and act on their
environments by means of DNA-based protein control.
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