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2017 ; 17
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English Wikipedia
DNA Sequencing Sensors: An Overview
#MMPMID28335417
Garrido-Cardenas JA
; Garcia-Maroto F
; Alvarez-Bermejo JA
; Manzano-Agugliaro F
Sensors (Basel)
2017[Mar]; 17
(3
): ä PMID28335417
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The first sequencing of a complete genome was published forty years ago by the
double Nobel Prize in Chemistry winner Frederick Sanger. That corresponded to the
small sized genome of a bacteriophage, but since then there have been many
complex organisms whose DNA have been sequenced. This was possible thanks to
continuous advances in the fields of biochemistry and molecular genetics, but
also in other areas such as nanotechnology and computing. Nowadays, sequencing
sensors based on genetic material have little to do with those used by Sanger.
The emergence of mass sequencing sensors, or new generation sequencing (NGS)
meant a quantitative leap both in the volume of genetic material that was able to
be sequenced in each trial, as well as in the time per run and its cost. One can
envisage that incoming technologies, already known as fourth generation
sequencing, will continue to cheapen the trials by increasing DNA reading lengths
in each run. All of this would be impossible without sensors and detection
systems becoming smaller and more precise. This article provides a comprehensive
overview on sensors for DNA sequencing developed within the last 40 years.