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2016 ; 5
(1
): ä Nephropedia Template TP
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DNA Methylation Analysis: Choosing the Right Method
#MMPMID26751487
Kurdyukov S
; Bullock M
Biology (Basel)
2016[Jan]; 5
(1
): ä PMID26751487
show ga
In the burgeoning field of epigenetics, there are several methods available to
determine the methylation status of DNA samples. However, choosing the method
that is best suited to answering a particular biological question still proves to
be a difficult task. This review aims to provide biologists, particularly those
new to the field of epigenetics, with a simple algorithm to help guide them in
the selection of the most appropriate assay to meet their research needs. First
of all, we have separated all methods into two categories: those that are used
for: (1) the discovery of unknown epigenetic changes; and (2) the assessment of
DNA methylation within particular regulatory regions/genes of interest. The
techniques are then scrutinized and ranked according to their robustness, high
throughput capabilities and cost. This review includes the majority of methods
available to date, but with a particular focus on commercially available kits or
other simple and straightforward solutions that have proven to be useful.
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