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10.1210/me.2015-1059 http://scihub22266oqcxt.onion/10.1210/me.2015-1059 C4484783!4484783!26038943     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26038943.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Endocrinol 2015 ; 29 (7): 1067-79 Nephropedia Template TP {{tp|p=26038943|t=2015. DICER Inactivation Identifies Pancreatic ?-Cell ?Disallowed? Genes Targeted by MicroRNAs |pdf=|usr=}}{{26038943}} gab.com Text DICER Inactivation Identifies Pancreatic -Cell Disallowed Genes Targeted by MicroRNAs JO: Mol Endocrinol http://www.kidney.de/pget.php?&tw=1&pmid=26038943 #FOAvip Pancreatic ?-cells are the body's sole source of circulating insulin and essential for the maintenance of blood glucose homeostasis. Levels of up to 66 ?disallowed? genes, which are strongly expressed and play housekeeping roles in most other mammalian tissues, are unusually low in ?-cells. The molecular mechanisms involved in repressing these genes are largely unknown. Here, we explore the role in gene disallowance of microRNAs (miRNAs), a type of small noncoding RNAs that silence gene expression at the posttranscriptional level and are essential for ?-cell development and function. To selectively deplete miRNAs from adult ?-cells, the miRNA-processing enzyme DICER was inactivated by deletion of the RNase III domain with a tamoxifen-inducible Pdx1CreER transgene. In this model, ?-cell dysfunction was apparent 2 weeks after recombination and preceded a decrease in insulin content and loss of ?-cell mass. Of the 14 disallowed genes studied, quantitative RT-quantitative real-time PCR revealed that 6 genes (Fcgrt, Igfbp4, Maf, Oat, Pdgfra, and Slc16a1) were up-regulated (1.4- to 2.1-fold, P < .05) at this early stage. Expression of luciferase constructs bearing the 3?-untranslated regions of the corresponding mRNAs in wild-type or DICER-null ?-cells demonstrated that Fcgrt, Oat, and Pdgfra are miRNA direct targets. We thus reveal a role for miRNAs in the regulation of disallowed genes in ?-cells and provide evidence for a novel means through which noncoding RNAs control the functional identity of these cells independently of actions on ?-cell mass. Twit Text FOAVip DICER Inactivation Identifies Pancreatic -Cell Disallowed Genes Targeted by MicroRNAs ????Mol Endocrinol http://www.kidney.de/pget.php?&tw=1&pmid=26038943 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26038943 #FOAvip English Wikipedia <ref>{{Cite pmid|26038943}}</ref> DICER Inactivation Identifies Pancreatic ?-Cell ?Disallowed? Genes Targeted by MicroRNAs #MMPMID26038943 Martinez-Sanchez A; Nguyen-Tu MS; Rutter GA Mol Endocrinol 2015[Jul]; 29 (7): 1067-79 PMID26038943show ga Pancreatic ?-cells are the body's sole source of circulating insulin and essential for the maintenance of blood glucose homeostasis. Levels of up to 66 ?disallowed? genes, which are strongly expressed and play housekeeping roles in most other mammalian tissues, are unusually low in ?-cells. The molecular mechanisms involved in repressing these genes are largely unknown. Here, we explore the role in gene disallowance of microRNAs (miRNAs), a type of small noncoding RNAs that silence gene expression at the posttranscriptional level and are essential for ?-cell development and function. To selectively deplete miRNAs from adult ?-cells, the miRNA-processing enzyme DICER was inactivated by deletion of the RNase III domain with a tamoxifen-inducible Pdx1CreER transgene. In this model, ?-cell dysfunction was apparent 2 weeks after recombination and preceded a decrease in insulin content and loss of ?-cell mass. Of the 14 disallowed genes studied, quantitative RT-quantitative real-time PCR revealed that 6 genes (Fcgrt, Igfbp4, Maf, Oat, Pdgfra, and Slc16a1) were up-regulated (1.4- to 2.1-fold, P < .05) at this early stage. Expression of luciferase constructs bearing the 3?-untranslated regions of the corresponding mRNAs in wild-type or DICER-null ?-cells demonstrated that Fcgrt, Oat, and Pdgfra are miRNA direct targets. We thus reveal a role for miRNAs in the regulation of disallowed genes in ?-cells and provide evidence for a novel means through which noncoding RNAs control the functional identity of these cells independently of actions on ?-cell mass. äDICER Inactivation Identifies Pancreatic ?-Cell ?Disallowed? Genes Tar(epmc).pdf DeepDyve Pubget Overpricing