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2015 ; 407
(10
): 2803-9
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Cytoskeleton dynamics in drug-treated platelets
#MMPMID25701419
Finkenstaedt-Quinn SA
; Ge S
; Haynes CL
Anal Bioanal Chem
2015[Apr]; 407
(10
): 2803-9
PMID25701419
show ga
Platelet activation is a key process in blood clot formation. During activation,
platelets go through both chemical and physical changes, including secretion of
chemical messengers and cellular shape change. Platelet shape change is mediated
by the two major cytoskeletal elements in platelets, the actin matrix and
microtubule ring. Most studies to date have evaluated these structures
qualitatively, whereas this paper aims to provide a quantitative method of
examining changes in these structures by fluorescently labeling the element of
interest and performing single cell image analysis. The method described herein
tracks the diameter of the microtubule ring and the circumference of the actin
matrix as they change over time. Platelets were incubated with a series of drugs
that interact with tubulin or actin, and the platelets were observed for
variation in shape change dynamics throughout the activation process. Differences
in shape change mechanics due to drug incubation were observable in each case.
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