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2013 ; 435
(1
): 179-86
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Cryo-electron tomography of bacterial viruses
#MMPMID23217626
Guerrero-Ferreira RC
; Wright ER
Virology
2013[Jan]; 435
(1
): 179-86
PMID23217626
show ga
Bacteriophage particles contain both simple and complex macromolecular
assemblages and machines that enable them to regulate the infection process under
diverse environmental conditions with a broad range of bacterial hosts. Recent
developments in cryo-electron tomography (cryo-ET) make it possible to observe
the interactions of bacteriophages with their host cells under native-state
conditions at unprecedented resolution and in three-dimensions. This review
describes the application of cryo-ET to studies of bacteriophage attachment,
genome ejection, assembly and egress. Current topics of investigation and future
directions in the field are also discussed.