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10.21769/BioProtoc.2320 http://scihub22266oqcxt.onion/10.21769/BioProtoc.2320 C5580966!5580966 !28868328     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28868328 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Bio+Protoc 2017 ; 7 (10 ): ä Nephropedia Template TP {{tp|p=28868328 |t=2017. Creating a RAW264 7 CRISPR-Cas9 Genome Wide Library |pdf=|usr=}}{{28868328 }} gab.com Text Creating a RAW264 7 CRISPR-Cas9 Genome Wide Library JO: Bio Protoc http://www.kidney.de/pget.php?&tw=1&pmid=28868328 #FOAvip The bacterial clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 genome editing tools are used in mammalian cells to knock-out specific genes of interest to elucidate gene function. The CRISPR-Cas9 system requires that the mammalian cell expresses Cas9 endonuclease, guide RNA (gRNA) to lead the endonuclease to the gene of interest, and the PAM sequence that links the Cas9 to the gRNA. CRISPR-Cas9 genome wide libraries are used to screen the effect of each gene in the genome on the cellular phenotype of interest, in an unbiased high-throughput manner. In this protocol, we describe our method of creating a CRISPR-Cas9 genome wide library in a transformed murine macrophage cell-line (RAW264.7). We have employed this library to identify novel mediators in the caspase-11 cell death pathway (Napier et al., 2016); however, this library can then be used to screen the importance of specific genes in multiple murine macrophage cellular pathways. Twit Text FOAVip Creating a RAW264 7 CRISPR-Cas9 Genome Wide Library ????Bio Protoc http://www.kidney.de/pget.php?&tw=1&pmid=28868328 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28868328 #FOAvip English Wikipedia <ref>{{Cite pmid|28868328 }}</ref> Creating a RAW264 7 CRISPR-Cas9 Genome Wide Library #MMPMID28868328 Napier BA ; Monack DM Bio Protoc 2017[May]; 7 (10 ): ä PMID28868328 show ga The bacterial clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 genome editing tools are used in mammalian cells to knock-out specific genes of interest to elucidate gene function. The CRISPR-Cas9 system requires that the mammalian cell expresses Cas9 endonuclease, guide RNA (gRNA) to lead the endonuclease to the gene of interest, and the PAM sequence that links the Cas9 to the gRNA. CRISPR-Cas9 genome wide libraries are used to screen the effect of each gene in the genome on the cellular phenotype of interest, in an unbiased high-throughput manner. In this protocol, we describe our method of creating a CRISPR-Cas9 genome wide library in a transformed murine macrophage cell-line (RAW264.7). We have employed this library to identify novel mediators in the caspase-11 cell death pathway (Napier et al., 2016); however, this library can then be used to screen the importance of specific genes in multiple murine macrophage cellular pathways. äCreating a RAW264 7 CRISPR-Cas9 Genome Wide Library (epmc).pdf DeepDyve Pubget Overpricing