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2014 ; 70
(ä): 12.36.1-10
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Correlative fluorescence and electron microscopy
#MMPMID25271959
Schirra RT Jr
; Zhang P
Curr Protoc Cytom
2014[Oct]; 70
(ä): 12.36.1-10
PMID25271959
show ga
Correlative fluorescence and electron microscopy (CFEM) is a multimodal technique
that combines dynamic and localization information from fluorescence methods with
ultrastructural data from electron microscopy, to give new information about how
cellular components change relative to the spatiotemporal dynamics within their
environment. In this review, we will discuss some of the basic techniques and
tools of the trade for utilizing this attractive research method, which is
becoming a very powerful tool for biology labs. The information obtained from
correlative methods has proven to be invaluable in creating consensus between the
two types of microscopy, extending the capability of each, and cutting the time
and expense associated with using each method separately for comparative
analysis. The realization of the advantages of these methods in cell biology has
led to rapid improvement in the protocols and has ushered in a new generation of
instruments to reach the next level of correlation--integration.